The neaSCOPE assesses composition and organization of proteins at the 10 -nm scale
IR-neaSCOPE + s provides a ready-to-use solution to study the composition of biological specimens or to analyze sample organization and morphology at the 10nm length scale . The combination of nano-FTIR spectroscopy and IR nanoscopy results in a more complete understanding of the interface structures or interaction mechanisms of organic matter like individual cells or viruses on the single particle level .
WBC protein absorption map
DNA in white blood cell ( WBC )
Nanoscale spectroscopy and nanoscopy of protein and DNA rich regions within a white blood cell ( WBC ) nucleus . The protein absorbance signature measured by nano-FTIR clearly shows the typical Amide I and Amide II bands . The DNA spectrum can be identified as a mixture of single- and double-stranded DNA . IR nanoscopy can be used to highlight the distribution of proteins and DNA within the nucleus . By directly resolving the nuclear organization of WBC nuclei , the neaSCOPE will open new possibilities to measure and to understand the significantly altered nuclear organization of e . g . classical Hodgkin ’ s Lymphoma .
DNA
2 µ m
Protein
WBC nano-FTIR absorption Amide I
Amide II
Amide I
δNH 2
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ν sym
PO 2
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ν asym
PO 2
-
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Amide II |
νC = O & νC = N |
1000 |
1200 |
1400 |
1600 |
1800 |
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Wavenumber [ cm -1 ] |
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G . C . Ajaezi et al ., Analyst 2018 , 143 , 5926
K . J . Kaltenecker et al ., Scientific Reports 2021 , 11 , 21860
Z . Qia K . Kanevche et al ., Communications Biology 2021 , 4 , 1341
G . Greaves et al ., Research Square 2023 , 25 , 2443252 nano-FTIR spectroscopy and IR nanoscopy disentangle the nuclear organization within white-blood cells .